Abstract
Background: Orthodontic treatment to improve aesthetics and for health reasons is performed in children and adults. Elderly individuals have low levels of estrogen, this results in alveolar bone resorption being greater than alveolar bone apposition. Isoflavones present in soybeans may be able to improve the remodeling process through the induction of osteoblastogenesis by increasing transforming growth factor-β1 (TGF-β1) levels. This study aimed to assess the comparative effect of soybean genistein isoflavone to TGF-β1 during orthodontic tooth movement among juvenile and adult rabbits. Methods: In this study, 12 healthy female rabbits were used. Subjects were divided into four groups (n=3); YG group (young rabbits), YGI group (young rabbits + isoflavones genistein), OG group (old rabbits), and OGI group (old rabbits + isoflavones genistein). Two lower incisors of the rabbit were moved distally using an orthodontic force (50 grams force) delivered by an open coil spring, which was inserted between two brackets. During active movements, the genistein isoflavones were given from the initial installation of the device until days 21, at a dose of 1.2 mg/kg BW once a day. Measurement of TGF-β levels were performed on days 1, 7, 14, 21 after appliance installation. TGF-β1 expression was analyzed using enzyme-linked immunosorbent assay (ELISA) and the optical density (OD) of the sample quantifed using a standard curve. The data obtained were analyzed using one-way Anova followed by Tukey HSD test. Results: The TGF-β1 levels were found to highest in the YGI group, and the TGF-β levels were significantly lower in the OG group ( p<0.05). ELISA analysis also revealed that TGF-β1 levels of the OGI group were significantly higher when compared with the OG group ( p<0.05). Conclusion: The administration of soybean genistein isoflavones could improve TGF-β1 levels in old rabbit's during active orthodontic tooth movement.
Highlights
Today’s society is witnessing an increased interest in cosmetic dentistry, thereby making orthodontics a necessity[1]
A previous study found that adults alveolar bone remodeling is much slower than in juveniles due to decreased cellular activity and vascularity, this suggests that orthodontic treatment duration may be longer in adults due to a delay in orthodontic tooth movement (OTM)[5]
This study aimed establish the comparative effect of genistein on transforming growth factor-β1 (TGF-β1) during orthodontic tooth movement among juvenile and adult rabbits
Summary
Today’s society is witnessing an increased interest in cosmetic dentistry, thereby making orthodontics a necessity[1]. The demand for orthodontic treatment has grown over time, in children, and in adults[4]. It would be better if you mentioned why you only examine the TGF-Beta only in this study?. Orthodontic treatment to improve aesthetics and for health reasons is performed in children and adults. Isoflavones present in soybeans may be able to improve the remodeling process through the induction of osteoblastogenesis by increasing transforming growth factor-β1 (TGF-β1) levels. This study aimed to assess the comparative effect of soybean genistein isoflavone to TGF-β1 during orthodontic tooth movement among juvenile and adult rabbits. ELISA analysis revealed that TGF-β1 levels of the OGI group were significantly higher when compared with the OG group (p
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