Abstract
Bio-organic fertilizers based on biocontrol microorganisms have been widely applied to suppress soilborne diseases and improve crop yields. Studies on beneficial biocontrol agents have promoted the development of the bio-organic fertilizers in China. Our previous study demonstrated that a biocontrol agent, Erythrobacter sp. YH-07, can inhibit the growth of the plant pathogen Fusarium oxysporum. In the present study, we investigated the effects of this biocontrol agent on tomato wilt and used the illumina-based sequencing approach to characterize the variations in soil bacterial communities in a potted experiment. The aim of our study was to explore the potential correlation among bacterial communities, Fusarium wilt suppression, and soil properties after application of the biocontrol agent YH-07. The results showed that application of Erythrobacter sp. YH-07 effectively controlled outbreaks of tomato Fusarium wilt. The illumina MiSeq sequencing showed that Proteobacteria was the predominant phylum in the soil samples. Bacterial community composition and structure varied under different soil treatments, e.g., the relative abundance of Erythrobacter and Salinimicrobium was significantly increased in the YH treatment, and Acidobacteria were decreased in the YH treatment compared with the CK treatment. Additionally, the correlation results showed that the soil organic matter and available phosphorus and potassium were higher after YH-07 application, and they were positively correlated with bacterial community. The redundancy analysis showed Erythrobacter and Acidobacteria were the dominant genera after YH and CK treatments, respectively, and correlations with tomato Fusarium wilt incidence were negative and positive, respectively.
Highlights
Tomato (Solanum lycopersicum L.) Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is common worldwide,[1] infecting the tomato roots, causing bacterial wilt, and limiting annual tomato yield.[2]
The incidence of tomato wilt disease in the YH treatment at 30, 45, and 60 days was 19.4%, 21.7%, and 27.4%, respectively, which was signi cantly lower than that in the CK treatment, in which disease incidence was 28.8%, 33.4%, and 48.6%, respectively, at the same time periods (Fig. 1). These results indicated that the biocontrol agent YH-07 more effectively controlled the outbreak of wilt disease in tomato plants
All the results indicated that short-term application of a biocontrol agent did not signi cantly affect the richness and diversity of bacterial communities, similar to a previous study reporting that the soil bacterial diversity as a result of bio-organic fertilizer (BIO) treatment was not the highest compared to other treatments, as determined by the Shannon index.[35]
Summary
Tomato (Solanum lycopersicum L.) Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is common worldwide,[1] infecting the tomato roots, causing bacterial wilt, and limiting annual tomato yield.[2]. Lycopersici (Fol) is common worldwide,[1] infecting the tomato roots, causing bacterial wilt, and limiting annual tomato yield.[2] Organic amendments, crop rotation, and chemical soil fumigation have been suggested for controlling the disease.[3,4] there is a risk that pathogens may become even more abundant in a short time.[5,6] Using antagonistic bacteria to prevent the disease may be the most promising control method, because biocontrol can provide ecosystem protection, food safety, and high efficiency.[7,8,9]. Microorganisms respond quickly to soil properties, fertilizer management, plant development, and other environmental factors.[14] Bonanomi et al.[5] suggested that the change in denitri cation with fertilizer management was related to the shi in bacterial communities resulting from variation in soil properties, which may cause long-term shi s in the quality and function of the soil.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
