Different cultivation media conditions affecting behavior and genetic stability of <i>Lepidium sativum</i> L.

Abstract

<i>Lepidium sativum</i> is an edible medicinal plant from the family Brassicaceae. It holds many uses in folk medicine, and the bioactivities activities originate from its constituents. So, it is essential to estimate the different physiological compounds in this plant. It is also essential to determine which organ in the plant could contain these active compounds. So, it is necessary to evaluate the growth conditions of this plant. In this study,<i> L. sativum</i> is grown in different conditions (with water only in Petri-dish, in pots with peat-moss soil and on jars with Murashige and Skoog (MS) medium with tissue culture technique). From the results obtained, it was clear that cultivation in peat-moss soil supplement the plant with more pigmentation concentration (but not for a long time) and much protein content. In comparison, cultivation on MS media provides much protein content. In all parameters, seeds germinated with water only have the lowest value. Genetically, RAPD-PCR molecular marker illustrates that there is no genetic stability in <i>L. sativum</i> grew on different cultivation conditions. The polymorphism percentage was 54.71%. So, there is genetic variation in response to different cultivation conditions.