Abstract
Previously, there have been few publications on free amino acid analysis in human tears. These studies used dated techniques based on paper chromatography. Because of their poor determination and the need of a high volume sample for analysis, all of the assays were done on reflex tear and produced, at best, only semi-quantitative results. To date, the role of tear free amino acids in the economy of the cornea and conjunctiva has not been studied, and no specific diseases have been reported in which qualitative or quantitative derangement of the tear amino acid composition is implicated.
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