Abstract
Chemical reagents that are used for synthesis of nanoparticles are often toxic, while biological reagents are safer and cost-effective. Here, the behavior of Staphylococcus epidermidis (ATCC 12228) was evaluated for biosynthesis of silver nanoparticles (Ag-NPs) and cadmium sulfide nanoparticles (CdS-NPs) using TEM images intra- and extracellularly. The bacteria only biosynthesized the nanoparticles intracellularly and distributed Ag-NPs throughout the cytoplasm and on outside surface of cell walls, while CdS-NPs only formed in cytoplasm near the cell wall. A new method for purification of the nanoparticles was used. TEM images of pure CdS-NPs confirmed biosynthesis of agglomerated nanoparticles. Biosynthetic Ag-NPs were more stable against bright light and aggregation reaction than synthetic Ag-NPs (prepared chemically) also biosynthetic Ag-NPs displayed lower toxicity in in vitro assays. CdS-NPs indicated no toxicity in in vitro assays. Biosynthetic nanoparticles as product of the detoxification pathway may be safer and more stable for biosensors.
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