Abstract
Crystallography is more like an art than science. Crystallizing membrane proteins are a big challenge; membrane proteins are present in the cell membrane and serve as cell support. The most important feature of membrane protein is that it contains both hydrophobic and hydrophilic regions on its surface. They are generally much more difficult to study than soluble proteins. The problem becomes more difficult when trying to obtain crystals to determine the high resolution structures of membrane proteins. We want to utilize this opportunity to briefly examine various approaches for crystallization of membrane proteins. The important factors for determining the success of crystallization experiments for membrane proteins lies in the purification, preparation of membrane samples, the environment in which the crystals are grown and the technique used to grow the crystals. All the X-ray structures of membrane protein are grown from preparations of detergents by different methods developed to crystallize. In this review different techniques for the crystallization of membrane proteins are being described. The cubic phase method also known as in meso method is discussed along with other methods to understand about the crystallization of membrane proteins, its general applicability, salt, detergent and screening effects on crystallization. Low volumes as nano-liter of samples can be used for crystallization. The effects of different detergents on the crystallization of membrane protein, as well as the use of surfactants like polyoxyethylene. Approach based on the detergent complexation to prove the ability of cyclodextrins to remove detergent from ternary mixtures in order to get 2D crystals. Crystallization of membrane proteins using non-ionic surfactants as well as Lipidic sponge phase and with swollen lipidic mesophases is discussed to better understand the crystallization of membrane proteins.
Highlights
INTRODUCTIONThese factors make them very important from the study point of view and crystallography is the most
With the in meso method the size could be increased to include large membrane proteins and so the crystallization of the smaller proteins would get an acceleration in the cubic phase with larger aqueous channels, this can happen by inducing a swelling of the meso phase while maintaining the properties of a continuous lipid bilayer of the membrane protein
The main purpose here was to understand the phase transformation occurring during the crystallization experiment
Summary
These factors make them very important from the study point of view and crystallography is the most Membrane proteins are those that exist in the reliable way to explore membrane protein structurecell membrane and are essential for cellular life. They function relationship in atomic detail (Chiu et al, 2000). Contribute too many processes where by the cells interact with the environment and generate energy and maintain the cellular structure (Wiener, 2004). They serve as both active and passive channels for ions and chemicals. In this review new approaches with the in meso methods along with few other approaches have been studied so as to understand the different aspects of crystallizing membrane proteins and try to overcome the drawbacks which interfere with the formation of crystals (Cherezov et al, 2004)
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