Abstract
BackgroundImmunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. Each year the amount of data, received by IHC, grows in geometric progression. But the lack of standardization, especially on the post-analytical stage (interpreting and reporting of results), makes the comparison of the results of different studies impossible.MethodsComprehensive PubMED literature search with a combination of search words “immunohistochemistry” and “scoring system” was performed and 773 articles describing IHC results were identified. After further manual analysis 120 articles were selected for detailed evaluation of used approaches.ResultsSix major approaches to the interpretation and presentation of IHC analysis results were identified, analyzed and described.ConclusionsThe overview of the existing approaches in evaluation and interpretation of IHC data, which are provided in the article, can be used in bone tissue research and for either better understanding of existing scoring systems or developing a new one. Standard multiparametric, semiquantitative IHC scoring systems should simplify and clarify the process of interpretation and reporting of received data.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_221
Highlights
The main aim of any histopathological investigation is the identification of a pathological process, special diagnostic features are necessary
Immunohistochemistry is a well-established tool, which is widely used to help identifying a wide spectrum of specific pathological processes and which is used in experimental research involving bone tissue
Exemplary for Vascular Endothelial Growth Factor (VEGF) and Bone Morphogenic Proteins (BMP) the tendency in ongoing immunohistochemistry researches is shown in Figure 1 – their number is growing in geometric progression
Summary
The main aim of any histopathological investigation is the identification of a pathological process, special diagnostic features are necessary. Revealing of such features in bone tissue is concerned with several differences compared to other tissues. Immunohistochemistry is a well-established tool, which is widely used to help identifying a wide spectrum of specific pathological processes and which is used in experimental research involving bone tissue. Immunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. After further manual analysis 120 articles were selected for detailed evaluation of used approaches
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
