Different antioxidant regulation mechanisms in response to aluminum-induced oxidative stress in Eucalyptus species

Abstract

Forest ecosystems play an important role in environmental protection and maintaining ecological balance. Understanding the physiological mechanisms of tree species response to aluminum (Al) toxic is crucial to reveal the main causes of plantation decline in acid rain area. As an important afforestation tree species in tropical and subtropical areas, Eucalyptus has high economic value and plays crucial ecological roles. However, continuous fertilization and acid precipitation can exacerbate soil acidification and increase soil active Al, which has a significant negative impact on Eucalyptus growth. Hence, species and genotypes with high Al resistance are required to solve the problem of Al toxicity of acidic soils for sustainable forest production. In this study, E. urophylla was better adapted to Al stress than E. grandis or E. tereticornis; its high Al resistance was attributed to greater antioxidant enzyme activity and non-enzymatic antioxidant content, and a lower degree of membrane lipid peroxidation than E. grandis or E. tereticornis. The differences in adaptability among the three pure species were attributed to their distinct habitats. Eucalyptus urophylla × E. grandis inherited the outstanding adaptability to Al stress from its maternal species (E. urophylla), indicating that Al tolerance is highly heritable and can be selected in Eucalyptus breeding. Our results indicated that the response of Eucalyptus to Al stress may fluctuate according to the time under stress, and might be related to dynamic changes in ROS elimination and accumulation.