Different anti-inflammatory effects of Lactobacillus acidophilus and Bifidobactrum bifidioum in hepatocellular carcinoma cancer mouse through impact on microRNAs and their target genes

Abstract

Cancer is one of the most important causes of mortality in the world. General methods for cancer treatment have many side effects, while biological treatments such as probiotics consumption not only have no undesirable effects, but also are more acceptable method to treat the disease. Although probiotics have been recommended to therapy some diseases such inflammatory, infectious and neoplastic disorders, but their action mechanism is unknown. In this work, to investigate the inhibition effects of probiotics on Hepatocellular carcinoma and colorectal cancer progression, the genes involved in cancerous process were investigated in 38 Bulb/c mice. they divided into four groups including (I) Control (Healthy, without probiotic consumption) (II) Azoxymethane induced mice (III) AOM induced mice fed with Lactobacillus acidophilus, and (IV) AOM induced mice fed with Bifidobactrum bifidioum and the expression of four selected microRNAs and their target genes were analysed. The results showed that Azoxymethane, a potent colon carcinogen, treatment induced the expression of miR-221, miR-155 (in blood), Bcl-w and KRAS expression and decreased miR-122, PTEN and PU.1 expression in blood, but it has no effect on miR-18a in the liver tissue. The probiotic consumption enhanced miR-122 and PU.1 (in blood) as significant overexpression and down-regulated miR-221, miR-155 (in blood), Bcl-w and KRAS. Thus, the probiotics can help to control of cancer progression through postponing of metastasis process, reducing of inflammation and down and up-regulation of oncogenes/oncomirs and tumor suppressor genes/microRNAs, respectively.