Abstract
Samples of human costal and articular cartilage, varying in age from less than 20 to over 70 yr, were subjected to extraction by 0·02 m phosphate buffer and to digestion by crude α-amylase. Whereas hexosamine—and uronic acid—release from costal cartilage sharply decreased with age in both types of experiments, release of these substances from articular cartilage were virtually independent of age. Crude α-amylase-digestion resulted in a linear increase of the released hexosamine/uronic acid ratio with age from costal cartilage only. Both buffer extraction and enzyme digestion released slightly more collagen from senescent articular cartilage than from aged costal cartilage.
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