Abstract
According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%). However, we found a high-sugar type A. eriantha line (code was ‘MM24’, test material) during investigative process at Nancheng county (E 116° 48′, N 26° 23′, 845 m). We sheared its scions to asexual reproduction in Fengxin County (rootstock was A. deliciosa ‘Miliang 1’ with 7 years old) and at the same time DUS (Distinctness, Uniformity and Stability) test was also carried out. There were uncontested differences between the two comparative genotypes according to the results of polyacrylamide gel electrophoresis, it can be judged as a new cultivar. In addition, there was great similarity on most important morphological and quality characteristics. While, there was difference on SSC, DM and TS between the two materials on ripen fruit, these indicators were much higher on test material than on control. The sugar degree assessment showed that the sugar degree of test material was strong and retention time was long. Further, no sucrose was found before DAF 135 d in test material and sucrose were significantly higher than in control only at DAF 165 d and DAF 175 d. The qRT-PCR results of sucrose-related genes showed that the relative expression levels of AcSPS1, AcSPS3, AcSPS5 and AcSUS5 genes were consistent with the sucrose accumulation trend, which was probably the main genes for the difference in sugar degree.
Highlights
According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%)
We found a high-sugar type A. eriantha line in Nancheng county, Jiangxi province and its soluble solids content (SSC) of ripen fruit more than 19%, which was significantly higher than edible standard
We found that some characters of leaf and flower of ‘Ganlv 1’ were consist with ‘Ganmi 6’, such as shape of leaf, flower size, arrangement of petals and so on
Summary
According to the investigation of wild Actinidia eriantha in Jiangxi province of China, we found that soluble solids content of fruit was lower than edible standard (14%). A. eriantha is a unique germplasm resource in kiwifruit, the fruit has high nutrient, e.g., the content of AsA in the fruit of A. eriantha is 3 or 4 times higher than that of A. chinensis[3]. This novel berry fruit has very great development potential after currently most commercial planting of A. chinensis and A. deliciosa, the large abundant wild germplasm resources supply possible commercial cultivar selection for industry[4]. We found a high-sugar type A. eriantha line in Nancheng county, Jiangxi province and its soluble solids content (SSC) of ripen fruit more than 19%, which was significantly higher than edible standard. In this paper we introduced the main agronomic traits of fruit on high-sugar type A. eriantha line and determined the related genes expression, discussed the main reason on sugar types during fruit growth, it would lay a theoretical foundation for studying the regulatory mechanism of sugar accumulation
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