Differences of mandibular condylar cartilage thickness of Mus musculus after given different intensities of physical stress

Abstract

Objective: This study aimed to evaluate the differences in the thickness of Mus musculus mandible cartilage after being given low, moderate, and high-intensity physical stress. Materials and Methods: This research is an experimental laboratory study. A total of 12 Mus musculus were divided into four groups, which are the control group(C), low (LS), moderate(MS), and high-intensity physical stress group(HS). The physical stress group was given treatment with swimming exercises using 3%, 6%, and 9% of body weight with a duration of 40%, 70%, and maximum swimming time calculation. After the experiment was carried out, the animal was decapitated and deparaffinized using xylol to dissolve paraffin in tissue to make histopathology preparations that were stained with hematoxylin-eosin. The thickness of mandibular condylar cartilage was calculated under a light microscope with a magnification of 400x and analyzed using the One Way ANOVA test to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05). Results: The thickness of the mandibular cartilage was measured to be thinner in the MS group (56.30 ± 6.11) and the HS group (78.04 ± 19.31) compared to the C group 85,85 ± 7,20. Statistical analysis with One Way ANOVA test showed that all groups had a significant difference in the thickness of mandibular condylar cartilage (p=0.049). Conclusions: There was a difference in the thickness of the mandibular cartilage after giving low, moderate, and high-intensity physical stress.