Abstract
The objective of this study was to determine differences in chemical compositions according to fat deposition in the liver using an alcoholic fatty liver animal model and a high-fat diet-induced fatty liver animal model. A mouse model of chronic and binge ethanol feeding (NIAAA model), an alcoholic fatty liver model, was used to induce fatty liver according to the study protocol. The NIAAA control group had 15 mice. The NIAAA experimental group was administered with Lieber DeCarli diet powder. The high-fat diet control group was fed a general diet ad libitum. The high-fat diet fatty liver group was induced with a high-fat (60%) diet. Data were acquired by 9.4 T magnetic resonance spectroscopy for each fatty liver animal model. Fatty acids were calculated by quantifying each lipid proton through T2 correction. Difference in lipid proton (LP) for each model was identified with a multivariate statistical method. Mean differences in fatty acids among the four models were compared. The difference in LP composition was insignificant between the high-fat diet control and the experimental group. Orthogonal partial least squares discriminant analysis of the high-fat experimental group and the NIAAA experimental group showed no significant difference in the composition of each LP. However, there was a difference in the value of the composition deposited in the liver between NIAAA control and experimental groups. Comparison of each fatty acid between NIAAA control and experimental groups revealed that poly-unsaturated bond was significantly (p = 0.002) higher in the experimental group than in the control group. There were differences in total lipid and polyunsaturated bonds between NIAAA experimental and control groups. Methylene protons were deposited at lower concentrations whereas diallylic protons were deposited at higher concentrations in the NIAAA experimental group than in the control group.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.