Abstract
Yes-associated protein (YAP) has been identified as an oncoprotein that regulates organ size by modulating proliferative and apoptotic activities. The aim of this study was to measure YAP gene expression and protein levels during rat liver regeneration and in liver cancer, and to explore possible correlations between YAP levels and cancer cell proliferation following partial hepatectomy. We examined YAP expression in rat regenerating liver using real-time PCR, Western blotting and immunohistochemical methods. YAP mRNA and protein levels were measured in human hepatocellular carcinoma. The results demonstrated that YAP protein levels were markedly increased in the regenerating liver (2.5-3 times), while YAP mRNA levels decreased slightly (1.47-2.17 times) (P=0.017). YAP mRNA and protein levels were both higher in hepatocellular carcinoma than in para-cancerous tissue. In conclusion, liver regeneration alone elevated YAP protein levels without elevating YAP mRNA levels. YAP mRNA and protein levels were both elevated in liver cancer cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.