Abstract
Background: Bladder cancer is one of the most common malignancies. Advances have been made in diagnosis, therapy, and surveillance resulting in increasing survival rates of patients, but the search for more effective therapies is imperative to be continued. Among others, lectin-mediated targeting might be a promising concept. Thus, the potential of fluorescein-labeled plant lectins was investigated using 5637 cells as a model for human urinary carcinoma [1] and SV-HUC-1 cells as a model for non-tumorigenic human uroepithelial cells.
Highlights
Taxol is one of the most traded, powerful antimitotic drugs and can cause cellular arrest during the G2/M phase of various tumor cells, including in leukemia, breast, ovarian, and lung cancer cells [1]
The Taxadiene synthase (TDS) producing potency of the recovered bacterial isolates were assessed by being grown on PDB for 4 days at 30 ◦ C, and the cultures were pelleted and pulverized in liquid nitrogen, and the intracellular proteins were extracted, and the enzyme activity and concentrations were determined by the standard assay
The highest TDS activity was produced by bacterial isolate # (8.8 μmol/mg/min), followed by isolate # (4.7 μmol/mg/min), while TDS production ranged between 1.9–2.6 μmol/mg/min for bacterial isolates # 10, 37, 38, 39, and 40
Summary
Taxol is one of the most traded, powerful antimitotic drugs and can cause cellular arrest during the G2/M phase of various tumor cells, including in leukemia, breast, ovarian, and lung cancer cells [1]. The biochemistry of Taxol biosynthesis, and different strategies for commercial Taxol production from endophytic fungi and plants have been extensively reviewed [5,6,7,8,9,10]. Aspergillus flavipes and A. terreus, endophytes of Podocarpus gracilior, have been recognized as efficient Taxol producers for the initial cultures; their Taxol biosynthetic potency is strongly attenuated by subculturing and storage [5,10,14,15,16,17,18]. Taxol productivity by A. flavipes was completely restored upon cocultivation with Bacillus subtilis due to the production of specific chromatin remodeling signals that triggered the expression of the fungal biosynthetic genetic cluster of Taxol [9,16,19]
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