Abstract
Anti-macrophage sera (AMS) produced in rabbits against rat alveolar (AM) and peritonealmacrophages (PM) were tested for their selective capacity to kill 51Cr-labelled AM, PM and thymus lymphocytes (TL) of rats. All non-absorbed AMS were able to kill both types of macrophages as well as TL in thepresence of complement. However, the cytotoxic titre (CT) and the percent of specific 51Cr-release was always higher on homologous macrophages (as used for immunization) than on heterologous macrophages (of the other population). On both types of macrophages it was stronger than on TL. After absorptions of AMS with insolubilized rat plasma and fetal calf serum (FCS), with raterythrocytes and with non-adherent cells from rat kidney, thymus, spleen and bone marrow (PEKL-absorptions) all AMS reacted only with macrophages and not with TL. When these antisera were absorbed repeatedly with limited amounts of AM or PM afunctional specificity was observed for the cell type which was not used for absorption. Further absorptions of these AMS with the same cells removed completely all cytotoxic activity for both macrophage populations. It is supposed that AM and PM have at least two different antigenic determinants present ontheir surface but their density is different in the two cell populations. Antigens specific for one cell type could not be detected in complement dependent cytotoxicity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
