Differences in the Ca 2+ response resulting from neurotransmitter stimulations of rat parotid acini and ducts

Abstract

There are few data available regarding the differences in intracellular Ca 2+ responses of parotid acinar and ductal cells. This study investigated the Ca 2+ mobilization that was induced by the chemical stimulation of acinar and ductal cells from rat parotid glands. In fura-2 loaded parotid cells, carbachol increased the intracellular Ca 2+ concentration ([Ca 2+] i) to a greater extent in the acinar cells than in the ductal cells, but noradrenaline increased the [Ca 2+] i in the ductal cells more than in the acinar cells. Although there was no difference in the α1-adrenergic receptor agonist phenylephrine-induced Ca 2+ mobilization between acini and ducts, the β-adrenergic receptor agonist isoproterenol increased the [Ca 2+] i in only the ductal cells. Additionally, the effects of non-adrenergic, non-cholinergic neurotransmitters were investigated. Substance P and ATP increased the [Ca 2+] i in parotid acini and/or ducts. A substance P-induced Ca 2+ response was observed in only acini, while the ATP-induced Ca 2+ response was significantly higher in ducts than in acini. These results suggest that parotid acini have a greater sensitivity to cholinergic and substance P stimulation and a lesser sensitivity to β-adrenergic and ATP stimulation than the ductal cells. In light of these results, substance P and isoproterenol will be useful for identifying parotid acini and ducts, respectively.