Differences in pH between interstitial fluid and arterial blood in water-breathing and air-breathing vertebrates.

Abstract

Most cells are bathed by interstitial fluid, but extracellular pH measurements are mostly for arterial plasma. Whole-body mean pH differences between the two fluids have been estimated in terms of a simple model. This relates to the diffusive exchange of carbon dioxide and oxygen and utilizes literature data, for 22 vertebrate species, on arterial and mixed-venous tensions of both gases. Uncertainties arise because the carbon dioxide reaction in blood may sometimes be in disequilibrium and because carbon dioxide diffusion is facilitated to unknown degrees in the presence of buffers. Nevertheless, the model suggests that the pH difference should tend to vary inversely with arterial carbon dioxide tension. In some species, this may aid interstitial pH homeostasis, but a clearer implication is that the difference should be generally greater in water breathers than in air breathers. It has previously been found that arterial pH in water-breathing teleosts also tends to be higher than in air-breathing tetrapods (when allowance is made for temperature and plasma sodium concentration) and to a comparable extent. Thus, mean interstitial pH may be more nearly similar in the two groups than is arterial pH. Direct measurements of interstitial pH do not yet suffice to test the model.