Abstract
Objective To compare gene expression profiles between AFP negative and AFP positive hepatocellular carcinoma (HCC). Methods The analysis of mRNA expression was performed on 20 samples of AFP negative and 20 samples of AFP positive HCC associated with HBV infection through Agilent® Whole Human Genome Oligo Microarray. Bioinformatics analyses were used to identify genes and real time PCR was applied to verify microarray data. Results 352 differentially expressed genes in the two groups were identified through principal component analysis. 62 up-regulated genes in AFP negative HCC were found to be involved in cell apoptosis and programmed cell death, whereas 290 up-regulated genes in AFP positive HCC involved in mitosis and cell cycle. Two differentially expressed genes including LAMB3 and STARD5 were further tested through real time PCR, and the results were in high concordance with microarray results. Conclusion The gene expression profiles of AFP negative HCC were different from AFP positive HCC. This study suggests there are different molecular mechanisms in HCC development and progression with different AFP levels. Key words: Carcinoma, hepatocellular; Alpha-fetoproteins
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