Abstract

In a comparison of the cytoplasms of Normal (N) and Texas (T) cytoplasmic male-sterile, consistently higher total and specific β-amylase activity was found in 10-day-old leaves of N plants. This was evident in all nine lines tested although the lines differed markedly in total amylase activity ranging from a low of 2.7 to a high of 112.5 (mg maltose/g/hr). Statistically significant differences were confirmed between cytoplasms and between inbred lines in β-amylase activity. On the basis of the activity ratio of N/T, the analysis of variance did not show any interaction between cytoplasms and lines, β-amylase activities from leaves of B37 N and T plants were identical with respect to Km, pH optimum, temperature sensitivity and mobility on gel electrophoresis. This difference between the N and T cytoplasms indicates that the β-amylase, though originating from chromosomal genes, is influenced by the cytoplasm in its total and specific activity and that this is correlated with male sterility.

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