Differences in amino acid composition and heme electronic structure of the multiple monomeric hemoglobin components of Glycera dibranchiata

Abstract

The monomeric hemoglobin fraction from Glycera dibranchiata can be reproducibly separated into three major and two minor components. The amino acid compositions reveal small but significant differences between the major components. 1H-nuclear magnetic resonance spectra show that the heme electronic structures are significantly different and indicate substantial differences between the heme pocket structures of the component hemoglobins.