Abstract
Fibroblasts were grown from explants of normal gingiva and foreskin, and from walls of dentigerous, radicular and residual cysts, as well as keratocysts of basal-cell naevus syndrome and non-syndrome origin. Dentigerous-cyst fibroblast adhesion to poly- l-lysine-coated glass was unaffected by all adhesion-related glycoproteins. Chondroitin sulphate, fibronectin and heparan sulphate enhanced attachment of all other fibroblasts. Chondroitin-sulphate and fibronectin-enhanced adhesion was blocked by an arg-gly-asp peptide. Fibronectin, chondroitin sulphate and laminin all promoted collagen lattice contraction using normal gingival fibroblasts and low-serum media. Fibronectin had a greater effect than chondroitin sulphate and laminin. In media with standard serum, all cyst fibroblast lines examined demonstrated similar gel contraction curves with the exception of dentigerous cyst-derived fibroblasts, which contracted at decreased rates. Suppression of gel contraction was seen with dentigerous-cyst fibroblasts with all extracellular matrix glycoproteins and low serum. Dentigerous-cyst fibroblast attachment to glass and behaviour in gel lattices suggest that these cells express different functional attachment factors from other cyst fibroblast types.
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