Differences between predicted outer membrane proteins of Pasteurella multocida, Histophilus somni, and genotype 1 and 2 Mannheimia haemolytica strains isolated from cattle.

Emily L Wynn,Michael L Clawson

doi:10.1139/gen-2021-0038

Abstract

Common bacterial causes of bovine respiratory disease (BRD) include Histophilus somni, Mannheimia haemolytica, and Pasteurella multocida. Within M. haemolytica, two major genotypes are commonly found in cattle (1 and 2); however, genotype 2 strains are isolated from diseased lungs much more frequently than genotype 1 strains. Outer membrane proteins (OMPs) of H. somni, P. multocida, and genotype 2 M. haemolytica may be important factors for acquired host immunity. The predicted OMP differences between genotypes 1 and 2 M. haemolytica have been previously identified. In this study, we expanded the focus to include bovine-isolated strain genomes representing all three species and the two M. haemolytica genotypes. Reported here are the core genomes unique to each of them, core genomes shared between some or all combinations of the three species and two M. haemolytica genotypes, and predicted OMPs within these core genomes. The OMPs identified in this study are potential candidates for further studies and the development of interventions against BRD.

Highlights

Bovine respiratory disease (BRD) is a multifactorial disease that affects cattle worldwide, with severe animal health, well-being, and economic ramifications (Rice et al 2007)
A total of 206 genes were unique to H. somni, 581 were unique to M. haemolytica, 143 were unique to genotype 1 M. haemolytica, 104 were unique to genotype 2 M. haemolytica, and 346 were unique to P. multocida (Fig. 1A; File S21)
Three genes encoding predicted Outer membrane proteins (OMPs) were identified in the shared core genome of all three species: a TonBdependent receptor protein TBDR, an OMP assembly factor BamA, and an LPS assembly protein LptD (Fig. 1B; Files S3 and S41)

Summary

Introduction

Bovine respiratory disease (BRD) is a multifactorial disease that affects cattle worldwide, with severe animal health, well-being, and economic ramifications (Rice et al 2007). Histophilus somni, Mannheimia haemolytica, and Pasteurella multocida are all members of the Pasteurellaceae family and are common bacterial causes of BRD (Klima et al 2019; Klima et al 2020). These gram-negative bacteria are opportunistic pathogens that colonize the nasopharynx of healthy cattle and can invade their lungs and cause BRD during times of general poor health, stress, and viral.

Methods

Results

Conclusion