Abstract
Ethyl acetate extracts of 26 terrestrial and 16 marine derived Phoma spp. were analysed using RP-HPLC coupled with diode-array detection and thermospray mass spectrometry. For all samples, the retention times, UV and mass spectral data were determined. Twenty-two mass spectral data points from single fungal metabolites could be identified as being discriminative between the two groups of fungi and were analysed by regression analysis. The occurrence of these mass units allowed 84% of all of the extracts to be confirmed as being derived from either the terrestrial or the marine samples. This finding suggests that marine-derived Phoma spp. differ significantly from terrestrial Phoma spp. with respect to their secondary metabolite content. The number of detected secondary metabolites per strain, and the number of compounds per strain which were unique to the marine-derived Phoma spp. did not differ markedly from the findings with their terrestrial counterparts. Copyright © 2000 John Wiley & Sons, Ltd.
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