Difference of morphology and proliferation ability between diabetes mellitus-derived ADSCs and normal ADSCs

Abstract

Objective To investigate the morphological characteristics and proliferation ability of diabetes mellitus-derived adipose-derived stem cells (ADSCs) by comparing with normal-derived ADSCs. Methods The ADSCs could be achieved from diabetes mellitus' adipose tissue by the similar method of normal ADSCs isolation and culture, and then the differnce was compared between the two groups about their morphologies under microscope, and the proliferation ability of two groups was determined by CCK-8. Results ADSCs in patients with diabetes were obvious hypertrophy and irregular in morphology. A values of ADSCs in diabetes and non-diabetes were 0.210+ 0.002 and 0.390+ 0.006 in 1st day; 0.250+ 0.015 and 0.443+ 0.023 in 2nd day; 0.368+ 0.014 and 0.726+ 0.033 in 3rd day; 0.368+ 0.014 and 0.726+ 0.033 in 4th day; 1.767+ 0.072 and 3.153+ 0.067 in 5th day and 1.810+ 0.072 and 3.170+ 0.021 in 6th day, respectively. The difference was statstically significant at the beginning from 4th day (P<0.05) . Conclusions There is obvious difference of the microscopic morphology between the diabetes mellitus-derived ADSCs and normal-derived ADSCs. The proliferation ability of the diabetes mellitus-derived ADSCs is lower than normal-derived ADSCs. These difference could be closely related to ulcerous non-healing wounds in patients with diabetes mellitus. Key words: Adipose-derived stem cells; Diabetes mellitus; Proliferation ability; Morphological characteristics; Non-healing wounds