Difference in microRNA levels in the post-mortem blood from different sampling sites: A proof of concept

Abstract

MicroRNAs have various characteristics, including stability, and tissue and disease specificity. One of the important issues in forensics is that biomarkers can differ quantitatively depending on the sampling site. We aimed at evaluating the differences in microRNA levels in the post-mortem (PM) blood from different sampling sites.The study subjects comprised seventeen males and eleven females. The number of cardiac deaths (CD) and non-cardiac deaths (NCD) was eleven and seventeen, respectively. A cardiopulmonary resuscitation (CPR) was performed in fifteen cases. Venous blood was obtained from the external iliac vein for peripheral blood (PB), inferior vena cava for pre-cardiac blood (CB), and coronary sinus for post-cardiac blood (coronary sinus blood, CSB). The selected target microRNAs were cardiac-specific (miR208 and miR-1) and non-cardiac microRNAs (miR-16 and let-7e).The levels of miR-208b and miR-1 in the PM blood differed according to its sampling site and showed an increasing order of tendency in the PB, CB, and CSB. However, these variations according to sampling sites did not correlate with the post-mortem interval and the levels did not differ between the CD and NCD groups, and between the CPR and non-CPR groups. MiR-16 and let-7e levels did not vary according to the sampling site.The present study confirms that the cardiac-specific microRNA levels in the PM blood from different sampling sites are different. In addition, this study showed that the characteristics of target microRNAs, such as tissue-specificity, should be considered and the sampling site for microRNAs should be presented in forensic applications.