Difference in expression between AQP1 and AQP5 in porcine endometrium and myometrium in response to steroid hormones, oxytocin, arachidonic acid, forskolin and cAMP during the mid-luteal phase of the estrous cycle and luteolysis.

Agnieszka Skowronska,Patrycja Mlotkowska,Mariusz T Skowronski,Soren Nielsen

doi:10.1186/s12958-015-0128-7

Agnieszka Skowronska, Patrycja Mlotkowska + Show 2 more

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https://doi.org/10.1186/s12958-015-0128-7

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Abstract

BackgroundRecently, we demonstrated in vitro that AQP1 and AQP5 in the porcine uterus are regulated by steroid hormones (P4, E2), arachidonic acid (AA), forskolin (FSK) and cAMP during the estrous cycle. However, the potential of the porcine separated uterine tissues, the endometrium and myometrium, to express these AQPs remains unknown. Thus, in this study, the responses of AQP1 and AQP5 to P4, E2 oxytocin (OT), AA, FSK and cAMP in the porcine endometrium and myometrium were examined during the mid-luteal phase of the estrous cycle and luteolysis.MethodsReal-time PCR and western blot analysis.ResultsProgesterone up-regulated the expression of AQP1/AQP5 mRNAs and proteins in the endometrium and myometrium, especially during luteolysis. Similarly, E2 also stimulated the expression of both AQPs, but only in the endometrium. AA led to the upregulation of AQP1/AQP5 in the endometrium during luteolysis. In turn, OT increased the expression of AQP1/AQP5 mRNAs and proteins in the myometrium during mid-luteal phase. Moreover, a stimulatory effect of forskolin and cAMP on the expression of AQP1/AQP5 mRNAs and proteins in the endometrium and myometrium dominated during luteolysis, but during the mid-luteal phase their influence on the expression of these AQPs was differentiated depending on the type of tissue and the incubation duration.ConclusionsThese results seem to indicate that uterine tissues; endometrium and myometrium, exhibit their own AQP expression profiles in response to examined factors. Moreover, the responses of AQP1/AQP5 at mRNA and protein levels to the studied factors in the endometrium and myometrium are more pronounced during luteolysis. This suggests that the above effects of the studied factors are connected with morphological and physiological changes taking place in the pig uterus during the estrous cycle.

Highlights

We demonstrated in vitro that AQP1 and AQP5 in the porcine uterus are regulated by steroid hormones (P4, E2), arachidonic acid (AA), forskolin (FSK) and cAMP during the estrous cycle
We described the response of AQPs (AQP1 and AQP5) to treatments with steroids, OT, arachidonic acid (AA), forskolin (FSK) and cAMP in the uterine explants from cyclic gilts during the mid-luteal phase (Days 10–12) and luteolysis (Days 14–16) [32]
The expression of AQP1 was strongly elevated in response to P4, E2, OT, AA, FSK and cAMP (p

Summary

Introduction

We demonstrated in vitro that AQP1 and AQP5 in the porcine uterus are regulated by steroid hormones (P4, E2), arachidonic acid (AA), forskolin (FSK) and cAMP during the estrous cycle. The potential of the porcine separated uterine tissues, the endometrium and myometrium, to express these AQPs remains unknown. In this study, the responses of AQP1 and AQP5 to P4, E2 oxytocin (OT), AA, FSK and cAMP in the porcine endometrium and myometrium were examined during the mid-luteal phase of the estrous cycle and luteolysis. On the basis of in vitro studies, Franczak and Kotwica [4], and Wojciechowicz et al [5] reported that both porcine endometrium and myometrium are steroidogenic tissues producing progesterone, estrogens and androgens. As a result of ovarian steroid action, the uterine glands expand and the secretory activity increases, becoming the highest at the end of the secretory phase, while during luteolysis, under the influence of oxytocin (OT), uterine fluids and unnecessary cell debris are excreted [11, 12]

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