Dietary Ziziphus jujuba Fruit Influence on Aberrant Crypt Formation and Blood Cells in Colitis-Associated Colorectal Cancer in Mice.

Srinivasan Periasamy,Ming-Yie Liu,Chung-Teng Liu,Wang-Hung Wu,Se-Ping Chien

doi:10.7314/apjcp.2015.16.17.7561

Abstract

Ziziphus jujuba (ZJ) fruit is rich in bioactive functional components such as polysaccharides, triterpenoid acid, flavonoids and oleamide. It has been commonly used in the treatment of various diseases including diabetes, digestive disorders, diarrhea, skin infections, liver and urinary complaints. However, dietary effects with regard to chemoprevention of colon cancer have not been studied. The present study was performed to evaluate the protective effects of dietary ZJ against colitis-associated colon carcinogenesis in azoxymethane (AOM)-dextran sodium sulphate (DSS)-treated mice. AOM was injected (10 mg/kg b.wt., i.p.) and three cycles of 2% DSS in drinking water for 7 days with 14 days of normal drinking water in-between were administered to induce colitis-associated colon cancer. ZJ fruit was supplemented into feed at levels of 5 and 10%. Dietary ZJ significantly attenuated aberrant crypt foci (ACF) formation and also decreased the progression of hyperplasia to dysplasia. In addition, it significantly reduced circulating white blood cells, lymphocytes, neutrophils, monocytes, eosinophils, basophils and platelets compared to colon cancer mice. We conclude that ZJ supplementation may delay the progression of colon cancer from hyperplasia to dysplasia and ultimately adenocarcinoma and cancer. In addition, it decreased circulating tumor-related leukocytes, main regulators of cancer inflammation. Dietary consumption of ZJ fruit attenuated the formation of ACF and delayed the progression of colon cancer.

Highlights

Cancer is the most important public health burden around the globe
We aimed to investigate the effect of dietary Ziziphus jujuba (ZJ) against colitis-associated colon cancer
Dietary ZJ attenuated dysplastic aberrant crypt foci (ACF) ACF formation can be classified into three grades with hyperplasia, low grade dysplasia and high grade dysplasia

Summary

Introduction

Cancer is the most important public health burden around the globe. Colon cancer is one of the most common malignancies in many parts of the world (Abdull Razis and Noor, 2013). The incidence of CRC in the developing countries is rapidly increasing, due to alteration in life style (Siegel et al, 2013). It is one of the leading causes of cancer death worldwide and is the third most common form of malignancy in both men and women (Hamiza et al, 2012). CRC ranks the third place of cancer overall and the second leading cause of cancer-related death affecting both males and females in Europe (Siegel et al, 2013; Stotz et al, 2014)

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