Abstract
Summary Two 8-week growth trials were conducted to determine total aromatic amino acid requirement and tyrosine replacement value for phenylalanine in Cirrhinus mrigala fingerlings. To determine the phenylalanine requirement, 20 fish were randomly stocked in triplicate groups in 55-L indoor polyvinyl flow-through circular tanks and fed six experimental diets containing graded levels of phenylalanine (5.0, 7.5, 10.0, 12.5, 15.0 and 17.5 g kg−1, dry diet) with 10 g kg−1 tyrosine. Maximum weight gain (287%), best FCR (1.44) and PER (1.74) occurred at 12.5 g kg−1 dietary phenylalanine. Quadratic regression analysis of weight gain, FCR and PER data indicated phenylalanine requirement at 13.5, 12.9 and 12.7 g kg−1 of dry diet, respectively. Protein deposition was significantly (P < 0.05) higher at 12.5 g kg−1 dietary phenylalanine. Based on the above results, phenylalanine requirement of C. mrigala is recommended at 13.0 g kg−1 of dry diet, corresponding to 32.5 g kg−1 of protein. On the basis of the above requirement, a second experiment with a similar design was conducted using six diets containing graded levels of tyrosine (2.1, 4.0, 6.0, 8.0, 10.0 and 12.0 g kg−1) with 13.0 g kg−1 phenylalanine fixed in all diets to determine the phenylalanine replacement value with that of tyrosine. Maximum weight gain (315%), best FCR (1.47) and PER (1.69) was at 8.0 g kg−1 dietary tyrosine. Quadratic regression analysis of weight gain, FCR and PER data indicated tyrosine requirement at 9.0, 8.4 and 8.2 g kg−1 of dry diet, respectively. Protein deposition was significantly (P < 0.05) higher at 8.0 g kg−1 dietary tyrosine. On the basis of the above results, 8.5 g kg−1 tyrosine, corresponding to 21.3 g kg−1 of protein, is taken as the optimum requirement and the replacement value is 39.53% on a weight and 36% on a molar basis. Thus, the total aromatic amino acid requirement is 21.5 g kg−1 of diet, corresponding to 53.8 g kg−1 of protein for optimum C. mrigala growth.
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