Dietary supplementation with flaxseed oil as source of Omega-3 fatty acids improves seminal quality and reproductive performance in aged broiler breeder roosters

Abstract

Reproductive performance in aged broiler breeder roosters is not desirable. The purpose of this study was to assess the effects of dietary flaxseed oil and vitamin E on the semen parameters, hormonal profiles, fatty acid analysis of sperm, and fertility performance of aged roosters. Twenty four roosters were assigned into four treatments and received their diets as following: 1) basal diet as control group (CTRL), 2) basal diet supplemented with 200 mg/kg vitamin E (CTRL + VITE), 3) basal diet supplemented with 2% flaxseed oil (FLAX) and 4) basal diet supplemented with 2% flaxseed oil plus 200 mg/kg vitamin E (FLAX + VITE). Roosters were fed their diets for 60 days and then, different characteristics of reproduction in the roosters were examined during experiment. Different diets affected semen parameters (P ≤ 0.05) except semen volume and morphology. Various characteristics of semen were significant (P ≤ 0.05) during different times of experiment excluding the total motility, membrane integrity and morphology. The higher percentage of sperm concentration, total motility, progressive motility, viability and membrane integrity accorded with a lower lipid peroxidation were observed in the roosters fed diet of FLAX + VITE (P ≤ 0.05). A higher concentration of testosterone was detected in roosters fed FALX + VITE at day 60 (P ≤ 0.05). Moreover, the percentage docosapantaenoic acid (DPA), docosahexaenoic acid (DHA) of sperm were increased in the diet of FLAX (P ≤ 0.05). Finally, rate of fertility after artificial insemination was significantly higher in the diet of FLAX + VITE. It seems that supplementation of aged roosters’ diet with flaxseed oil and VITE improves the semen performance and fertility potential that can be a suitable strategy to preserve the reproductive performance of aged rooters.