Dietary supplementation with barley sprouts and d-aspartic acid improves reproductive hormone concentrations, testicular histology, antioxidant status, and mRNA expressions of apoptosis-related genes in aged broiler breeder roosters

Abstract

The objective was to determine effects of dietary supplementation of barley sprouts (BS) and/or d-aspartic acid (DA) on the reproductive potential of aged broiler roosters. Birds (n = 32, 50 wk old) were randomly allocated to receive dietary supplements of BS powder (2 % of basal diet), and DA (200 mg/kg BW), both, or neither, for 12 wk. Roosters were housed individually, with 14-h light/10-h dark, ad libitum feed and water, and euthanized after 12 wk. Mean (±SEM) total phenolic compounds and IC50 in methanol extract of sprouted barley were 302.8 ± 10.9 mg GAE/g and 600.8 ± 50.5 mg TE per 100 g, respectively. In weekly semen collections, sperm total and progressive motility, plasma membrane integrity, sperm concentration, and sperm production were higher (P < 0.05) in both the DA + BS and BS groups compared to the Control, but sperm abnormalities were unaffected. Feeding DA increased right, left, and combined testicular weights (P < 0.05, P < 0.05, and P < 0.01, respectively) and, the testicular index (P = 0.01). Feeding BS increased seminiferous tubule diameter (P < 0.01), whereas BS + DA increased seminiferous epithelium thickness (P < 0.01). There were more spermatogonia (P < 0.01) and Leydig cells (P < 0.05) in BS-fed roosters but Sertoli cells were highest in BS + DA (P < 0.01). Serum MDA concentrations were lowest in BS (P < 0.01), whereas serum testosterone and LH were highest in DA (P < 0.05) and BS + DA (P < 0.01), respectively. Feeding BS reduced serum total cholesterol (P < 0.05) and increased serum HDL-cholesterol (P < 0.01), with decreases in serum LDL (P < 0.01) and the LDL/HDL ratio (P < 0.01) for BS + DA compared to Control. Relative expression of glutathione peroxidase mRNA was increased by BS (P < 0.01) or DA (P < 0.05), whereas relative mRNA expression of SOD was highest in BA (P < 0.01). Control roosters were highest for both BAX (P < 0.01) and the relative expression of the BAX/BCL-2 ratio (P < 0.01), whereas BS + DA increased BCL-2 (P < 0.05). In conclusion, feeding BS, and/or DA significantly improved reproductive potential in aged broiler roosters.