Dietary solutions for aluminum embryotoxicity: A study in Danio rerio using spirulina and okra-spirulina diets

Abstract

Aluminum (Al) is a versatile element commonly employed in various industries and water treatment processes. However, its presence in aquatic ecosystems can elicit adverse effects on organisms, particularly the Danio rerio fish species. Aluminum exposure has been associated with a spectrum of issues, ranging from oxidative stress to behavioral anomalies, reproductive disruptions, and morphological alterations in these organisms. This research aimed to assess the impact of aluminum chloride (AlCl3) on D. rerio embryos and explore strategies to mitigate its effects. Three dietary groups (commercial, okra-spirulina, and spirulina) were studied, focusing on embryonic development, oxidative damage, and gene expression changes. The study revealed that diets enriched with spirulina and okra-spirulina effectively reduced aluminum-induced embryotoxicity, oxidative stress, and gene expression alterations, surpassing the commercial diet. However, all AlCl3-exposed groups experienced adverse effects on embryonic development, including hatching anomalies, structural deformities, and cardiac delays. The okra-spirulina group showed milder toxic responses. In conclusion, this study highlights the potential of spirulina and okra-spirulina diets in mitigating aluminum-triggered oxidative stress and apoptosis in D. rerio. It underscores the need for future research on embryonic development and carries significant implications for environmental conservation and the well-being of aquatic organisms in aluminum-contaminated environments.