Abstract
A 12-week growth trial was conducted in a flow-through system to determine dietary selenium (Se) requirement for on-growing gibel carp (initial body weight: 76.2 +/- 0.05 g, mean +/- SEM). Selenomethionine was supplemented to the basal diet to formulate seven semi-purified diets containing 0.26, 0.58, 0.72, 1.14, 1.34, 1.73 and 2.09 mg Se kg(-1) diet. The results showed that plasma superoxide dismutase (SOD) activity significantly increased when fish were fed with 0.58 mg Se kg(-1) diet (P = 0.72 mg kg(-1) (P > 0.05). Whole-body and muscle Se concentrations were higher when fed with 1.34 mg Se kg(-1) diet (P = 1.34 mg kg(-1) (P > 0.05). In conclusion, based on broken-line regression of hepatic Se concentrations, hepatic SOD activity and hepatic T-AOC activity, dietary Se requirements for on-growing gibel carp was 0.73 mg kg(-1), 1.12 mg kg(-1) and 1.19 mg kg(-1) diet respectively.
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