Dietary scopoletin modulates adipose tissue inflammation and lipid accumulation in chronic alcohol‐fed rats (647.12)

Abstract

Chronic alcohol consumption leads to lipolysis and reduction of fatty acid uptake capacity in the adipose tissue, which is associated with dysregulation of lipid metabolism. Therefore, we investigated the dose‐response effects of scopoletin on adipose tissue lipid homeostasis and inflammation in chronic alcohol‐fed rats. Rats were fed a Liber‐Decarli liquid diet contains 5% alcohol with or without three different doses of scopoletin (0.01g, 0.02g, 0.05g/L) for 8 weeks. Normal rats were received isocaloric carbohydrate liquid diet. All doses of scopoletin did not affect the visceral fat mass, but they significantly lowered triglyceride levels compared with the alcohol control group. Scopoletin dose‐independently inhibited lipolysis target gene of adipose tissue, CIDEA, along with lipogenesis genes (PPARγ, SREBP and FASN), while it increased CPT gene level compared with the alcohol control group. Furthermore, all doses of scopoletin significantly down‐regulated mRNA levels of inflammatory markers such as TLR4, NFκB and TNFα as well as plasma TNFα and IL‐6 levels in alcohol‐fed rats. Our findings demonstrated that low‐dose of scopoletin modulated alcohol‐induced dysregulation of lipid metabolism and imflammatory response in the adipose tissue, which was mediated by regulation of TLR4 signaling pathway and lipid metabolism related gene expression.Grant Funding Source: Supported by the National Research Foundation of Korea (No. 2012R1A1A2041931).