Abstract
Fingerling channel catfish were fed diets supplemented with 0, 2, 4, 6, 8, 10 and 12 mg riboflavin/kg diet for 8 weeks in flowthrough aquaria at a water temperature of 29 ± 1 °C. Riboflavin deficient fish exhibited short body dwarfism, poor growth, anorexia and reduced hepatic D-amino acid oxidase activity (D-AAO). No other gross deficiency signs were observed. A level of 4 mg riboflavin/kg diet was sufficient to prevent the appearance of gross deficiency signs, but a higher dietary level was required in order to obtain maximal performance in terms of growth and hepatic D-AAO activity. Based on the results obtained by morphological parameters, hepatic D-AAO activity and broken-line regression analysis, the dietary riboflavin requirement for channel catfish was estimated to be 6 mg/kg diet.
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