Dietary protease modulates nutrient retention efficiency and hepatopancreatic protease activity in juvenile Chinese mitten crab Eriocheir sinensis

Abstract

An 8-week growth trial was conducted with juvenile Chinese mitten crab fed a high fishmeal (FM) diet (PC), a low FM diet (NC) and three other diets (NC125, NC150 and NC175) supplemented with 125, 150 and 175 mg/kg of a dietary protease. In the NC diets, a portion of FM (from PC diet) was replaced by a combination of plant proteins. All diets were isoproteic (404 ± 3 g/kg) and isoenergetic (18.6 ± 0.2 kJ/g). No differences were observed among treatments in most parameters except protein (PRE, %) and lipid (LRE, %) retention efficiencies, where LRE was the highest (37.9%) in NC175 followed by the PC. A linear regression demonstrated significant effects of protease on hepatopancreatic trypsin activity (HPRO, p = .001), and PRE (p = .05) and LRE (p = .014) but not on energy retention efficiency (ERE). Dietary protease level was positively correlated with HPRO and nutrient retention efficiency (p < .05). On the other hand, the specific growth rate (SGR) was positively correlated with portein deposition (PD), lipid deposition (LP) and recovered energy (RE) and negatively correlated with survival (p < .05). This study is first to demonstrate that mainly nutrient retention efficiencies are modulated by specific dietary enzymes in animals.