Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs.

Wan Ibrahim Izuddin,Ali Merzza Humam,Hooi Ling Foo,Anjas Asmara Samsudin,Teck Chwen Loh

doi:10.3390/antiox9030250

Wan Ibrahim Izuddin, Ali Merzza Humam + Show 3 more

Open Access

https://doi.org/10.3390/antiox9030250

Copy DOI

Abstract

Postbiotics from Lactobacillus plantarum have been reported to improve growth performance, nutrient utilization, immune status and gut health in livestock. However, there is scarce information on the antioxidant activity of postbiotics and its modulation of antioxidant activity and rumen barrier function in animals. We investigated the antioxidant activity of postbiotics from L. plantarum RG14, RG11 and TL1 and dietary effects in post-weaning lambs on serum and ruminal antioxidant activity, hepatic antioxidant enzymes and ruminal barrier function. Postbiotic RG14 showed the highest antioxidant activity in both 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay and was chosen to be evaluated in animal trials. Twelve post-weaning Dorper lambs were allotted to the control group and postbiotic group (0.9% (v/w) postbiotic RG14). The improvement in antioxidant activity of the postbiotic group was observed by greater glutathione peroxidase (GPX) in serum and ruminal fluid and lower serum TBARS. The findings were strengthened by the upregulation of hepatic GPX1, GPX4 and copper, zinc superoxide dismutase (Cu/Zn SOD) in the postbiotic group. Lambs received postbiotics had higher regulation of rumen barrier function through upregulation of tight junction protein (TJP), occludin (OCLD), claudin-1 (CLDN1) and CLDN4. The current study demonstrated that dietary postbiotics enhanced the serum and ruminal fluid antioxidant activity, reduced the serum lipid peroxidation and upregulated hepatic antioxidant enzymes and ruminal barrier function.

Highlights

The exploration in finding the in-feed additives as alternatives to the use of antibiotics in livestock as a growth promoter has created huge attention due to the emergence of antimicrobial resistance.Recently, postbiotics, which are the secondary metabolites of probiotic bacteria, have been reported as great potential to substitute the use of antibiotics in animal production
Among the postbiotic L. plantarum, RG14 strain showed highest antioxidant activity ABTS radical scavenging assay as compared with RG11 and TL1 (Table 3)
Because of the higher in total antioxidant activity, only postbiotic RG14 was chosen to be further evaluated in the feeding trial of post-weaning lambs

Summary

Introduction

Postbiotics, which are the secondary metabolites of probiotic bacteria, have been reported as great potential to substitute the use of antibiotics in animal production. The potential of postbiotics is ascribed to the existence of organic acids and antimicrobial compounds like bacteriocins, which possess inhibitory ability against important pathogenic bacteria including Escherichia coli, Listeria monocytogenes, Pediococcus acidilactici, Salmonella typhimurium and Vancomycin-resistant Enterococci (VRE) [1,2]. In broilers exposed to heat stress, postbiotics inclusion was shown to alleviate the heat stress effects by the greater growth performance, immune status and gut health [9]. Postbiotics use in ruminants was shown to improve the in vitro rumen fermentation and fiber degrading microbial population [10] and enhance growth performance, nutrient utilization, immune status and gut health in post-weaning lambs [11,12]

Objectives

Methods

Results

Discussion

Conclusion