Dietary nitrate supplementation augments cutaneous reflex vasodilation in healthy humans

Abstract

Beetroot juice (BRJ) has been shown to increase NO‐dependent dilation through both NOS‐dependent and independent pathways. We hypothesized BRJ supplementation would augment reflex cutaneous active vasodilation. Subjects were equipped with two microdialysis fibers on the forearm and randomly assigned as control (Ringer's) or NOS inhibition (20mM L‐NAME). Whole‐body heating was achieved via water‐perfused suits to raise core temperature (Tc; ingestible telemetric pill) 0.8°C. Maximal cutaneous vasodilation was reached by administering 54mM SNP and local heating to 43°C. Skin blood flow was measured via laser‐Doppler flowmetry and mean arterial pressure was measured; cutaneous vascular conductance (CVC) was calculated and expressed as %CVCmax. Subjects underwent heat stress pre‐ and post‐nitrate supplementation (3 days of BRJ: 5mM, .45g nitrates per day). BRJ increased the plateau CVC at control (pre: 55±3 vs. post: 77±5 %CVCmax) and L‐NAME (pre: 27±3 vs. post: 41±6 %CVCmax; p<0.05 for all conditions) sites. The %NO contribution increased from pre‐ (47±2 %CVCmax) to post‐BRJ (71±4 %CVCmax; p<0.05). These data suggest BRJ augments NO‐dependent component of reflex cutaneous vasodilation.