Dietary n-3 highly unsaturated fatty acids affect the biological and serum biochemical parameters, tissue fatty acid profile, antioxidation status and expression of lipid-metabolism-related genes in grass carp,Ctenopharyngodon idellus

Abstract

A 95-day feeding trial was conducted to determine the effects of n-3 highly unsaturated fatty acids (n-3 HUFA) on the growth, antioxidation and lipid metabolism in grass carp (Ctenopharyngodon idellus). Two isonitrogenous and isolipidic diets were formulated with either lard oil (LO) or fish oil (FO) as the main lipid source. The results showed that the intraperitoneal fat (IPF) ratio was significantly lower (P < 0.05) in FO group. The concentration of n-3 HUFA in muscle, hepatopancreas and IPF was significantly higher in FO group (P < 0.05). The serum low-density lipoprotein (LDL) content was significantly lower (P < 0.05), and glucose (GLU) content was significantly higher (P < 0.05) in FO group. The serum total superoxide dismutase (T-SOD) activity was significantly higher (P < 0.05) in FO group, consistent with the serum malondialdehyde (MDA) content. The gene expression of IPF fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), sterol regulatory element-binding protein (SREBP-1) and peroxisome proliferator-activated receptor γ (PPARγ) was significantly lower (P < 0.05) and that of peroxisome proliferator-activated receptor α (PPARα) was significantly higher (P < 0.05) in FO group compared with LO group. Similar trends were found in the hepatopancreas, except for PPARγ. It is suggested that n-3 HUFA could inhibit lipid accumulation in grass carp by affecting the expression of lipid-metabolism-related genes.