Dietary Lactobacillus casei K17 Improves Lipid Metabolism, Antioxidant Response, and Fillet Quality of Micropterus salmoides.

Jinsong Wang,Xiangjun Leng,Huiyu Fu,Lanming Chen,Shenghao Tian,Zhuoying Zhu

doi:10.3390/ani11092564

Jinsong Wang, Xiangjun Leng + Show 4 more

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https://doi.org/10.3390/ani11092564

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Abstract

Simple SummaryIn order to find effective antioxidants to improve the fleshy degeneration and liver tissue lesions of Micropterus salmoides that were fed artificial mixed feed, Lactobacillus casei K17, which displayed a high level of antioxidant activity in vitro and in vivo was selected in this study. The results indicated that after a trial for 69 days, live bacteria (LB), live bacteria protected by skim milk powder (MB), and dead bacteria were able to improve hemal and hepatic lipid metabolism and antioxidant response, reduce reactive oxygen species production, and protect Micropterus salmoides hepatic cells from injury, while LB and MB were also able to improve fillet quality. Therefore, Lactobacillus casei K17 might be a good alternative source of improving fillet quality and liver health in Micropterus salmoides.We previously demonstrated that Lactobacillus casei K17, isolated from Korean kimchi, has high antioxidant levels in vitro and in vivo. However, its effect on Micropterus salmoides is unknown. In this study, we investigated the impact of L. casei K17 supplementation on the lipid metabolism, antioxidant response, liver histology, and fillet quality of M. salmoides. We randomly assigned 450 M. salmoides (33.0 ± 0.5 g) to six diet groups for 69 days. The diets were as follows: 0.85% normal saline; 10% skim milk powder; 1 × 108 CFU/g live L. casei K17 (LB); 1 × 108 live L. casei K17 protected by skim milk powder (MB); 1 × 108 dead L. casei K17 (DB); and L. casei K17 fermentation supernatant. MB significantly improved the crude protein, total collagen, alkaline-insoluble collagen, fiber numbers, hardness, chewiness, and gumminess of M. salmoides fillets (p < 0.05). LB significantly improved crude protein and fiber numbers (p < 0.05). Furthermore, dietary supplementation with LB, MB, and DB maintained normal liver histology, preserved liver function, and increased hepatic and hemal antioxidant status by enhancing antioxidant enzyme activities. Meanwhile, the three diets also promoted lipid metabolism by increasing HDL-C effectiveness and reducing total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels in serum and liver tissues, indicating that dietary supplementation with DB, LB, and MB had hypolipidemic effects on M. salmoides. MB and LB significantly improved fillet quality and LB, MB, and DB improved hemal and hepatic lipid metabolism and antioxidant response and reduced reactive oxygen species production, protecting M. salmoides hepatic cells from injury.

Highlights

Micropterus salmoides is an important carnivorous native of freshwater lakes and small rivers in North America [1]
Chen et al reported that increased oxidative stress in M. salmoides that were fed oxidized lipids led to stimulated hepatic antioxidant defenses, vitamin E depletion in plasma and certain tissues, and pathological changes [5]
We found that L. casei K17 displayed high level of antioxidant activity in vitro

Summary

Introduction

Micropterus salmoides (largemouth bass) is an important carnivorous native of freshwater lakes and small rivers in North America [1]. It has been widely cultured in China because of its rapid growth, tender flesh, and high nutritional value and has become an economically valuable species of fish [2,3]. Chen et al reported that increased oxidative stress in M. salmoides that were fed oxidized lipids led to stimulated hepatic antioxidant defenses, vitamin E depletion in plasma and certain tissues, and pathological changes [5]. Yun et al reported that slight oxidation of these lipids in M. salmoides feed would negatively affect fish growth [6]

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