Dietary Intervention With Specific Non-digestible Oligosaccharide Mixtures Is Effective During Induction Of Murine Cow'S Milk Allergy But Not Suitable As Treatment In The Same Allergic Disease Model

Abstract

RationaleIn this study, the effects of dietary supplementation with specific non-digestible oligosaccharides (prebiotics) either as single components or in mixtures on the outcome of the murine allergic response when provided before and during oral sensitization with whey were investigated. In addition, the feasibility to treat allergic mice with these prebiotics was addressed.MethodsMice were fed diets containing 1% single prebiotics, combinations of scGOS (short chain galacto-oligosaccharides), lcFOS (long chain fructo- oligosaccharides) and/or pAOS (pectin-derived acidic oligosaccharides) or a control diet. The diets were provided two weeks prior to and during oral whey-sensitization (prevention) or for four weeks starting one week after the last sensitization (treatment). The acute allergic skin response (ITH) and antigen-induced anaphylaxis (AiA) were determined one hour after intradermal whey-challenge. Whey-IgE/G1/G2a and mouse mast cell protease-1 (mMCP-1) were determined in serum.ResultsIn the whey-sensitized preventive intervention groups, only combination of scGOS/lcFOS (9:1) and scGOS/lcFOS/pAOS (9:1:2) strongly reduced both ITH (p<0.001) and AiA compared to controls. scGOS/lcFOS/pAOS was most effective, whereas single components were not. mMCP-1 tended to be reduced in the whey-sensitized mice fed scGOS/lcFOS(/pAOS), although the whey-IgE/G1/G2a remained high. In the treatment groups, allergic disease was confirmed prior to the diet intervention by presence of whey-IgE, ITH and AiA. Four weeks of dietary intervention with scGOS/lcFOS(/pAOS) did not alter the allergenic response compared to controls.ConclusionsDietary supplementation with scGOS/lcFOS(/pAOS) provided as prevention reduced the allergic effector response. However, these diets were reluctant to treat allergy in the chosen intervention protocol in the murine model of IgE-mediated hypersensitivity. RationaleIn this study, the effects of dietary supplementation with specific non-digestible oligosaccharides (prebiotics) either as single components or in mixtures on the outcome of the murine allergic response when provided before and during oral sensitization with whey were investigated. In addition, the feasibility to treat allergic mice with these prebiotics was addressed. In this study, the effects of dietary supplementation with specific non-digestible oligosaccharides (prebiotics) either as single components or in mixtures on the outcome of the murine allergic response when provided before and during oral sensitization with whey were investigated. In addition, the feasibility to treat allergic mice with these prebiotics was addressed. MethodsMice were fed diets containing 1% single prebiotics, combinations of scGOS (short chain galacto-oligosaccharides), lcFOS (long chain fructo- oligosaccharides) and/or pAOS (pectin-derived acidic oligosaccharides) or a control diet. The diets were provided two weeks prior to and during oral whey-sensitization (prevention) or for four weeks starting one week after the last sensitization (treatment). The acute allergic skin response (ITH) and antigen-induced anaphylaxis (AiA) were determined one hour after intradermal whey-challenge. Whey-IgE/G1/G2a and mouse mast cell protease-1 (mMCP-1) were determined in serum. Mice were fed diets containing 1% single prebiotics, combinations of scGOS (short chain galacto-oligosaccharides), lcFOS (long chain fructo- oligosaccharides) and/or pAOS (pectin-derived acidic oligosaccharides) or a control diet. The diets were provided two weeks prior to and during oral whey-sensitization (prevention) or for four weeks starting one week after the last sensitization (treatment). The acute allergic skin response (ITH) and antigen-induced anaphylaxis (AiA) were determined one hour after intradermal whey-challenge. Whey-IgE/G1/G2a and mouse mast cell protease-1 (mMCP-1) were determined in serum. ResultsIn the whey-sensitized preventive intervention groups, only combination of scGOS/lcFOS (9:1) and scGOS/lcFOS/pAOS (9:1:2) strongly reduced both ITH (p<0.001) and AiA compared to controls. scGOS/lcFOS/pAOS was most effective, whereas single components were not. mMCP-1 tended to be reduced in the whey-sensitized mice fed scGOS/lcFOS(/pAOS), although the whey-IgE/G1/G2a remained high. In the treatment groups, allergic disease was confirmed prior to the diet intervention by presence of whey-IgE, ITH and AiA. Four weeks of dietary intervention with scGOS/lcFOS(/pAOS) did not alter the allergenic response compared to controls. In the whey-sensitized preventive intervention groups, only combination of scGOS/lcFOS (9:1) and scGOS/lcFOS/pAOS (9:1:2) strongly reduced both ITH (p<0.001) and AiA compared to controls. scGOS/lcFOS/pAOS was most effective, whereas single components were not. mMCP-1 tended to be reduced in the whey-sensitized mice fed scGOS/lcFOS(/pAOS), although the whey-IgE/G1/G2a remained high. In the treatment groups, allergic disease was confirmed prior to the diet intervention by presence of whey-IgE, ITH and AiA. Four weeks of dietary intervention with scGOS/lcFOS(/pAOS) did not alter the allergenic response compared to controls. ConclusionsDietary supplementation with scGOS/lcFOS(/pAOS) provided as prevention reduced the allergic effector response. However, these diets were reluctant to treat allergy in the chosen intervention protocol in the murine model of IgE-mediated hypersensitivity. Dietary supplementation with scGOS/lcFOS(/pAOS) provided as prevention reduced the allergic effector response. However, these diets were reluctant to treat allergy in the chosen intervention protocol in the murine model of IgE-mediated hypersensitivity.