Dietary Intervention With α-Amylase Inhibitor in White Kidney Beans Added Yogurt Modulated Gut Microbiota to Adjust Blood Glucose in Mice.

Shenli Wang,Haiying Yang,Shuangli Mi,Fazheng Ren,Chongye Guo,Zhikai Xing,Lishui Chen,Yunting Zhang,Meng Li

doi:10.3389/fnut.2021.664976

Abstract

White kidney beans contain α-amylase inhibitors that can be used in diet for weight reduction. In this study, we investigated the potential of white kidney bean (phaseolus vulgaris L.) extract enriched in α-amylase inhibitor as a food additive in yogurt to regulate blood glucose in hyperglycemic animals. Five groups of C57BL/6J mice were fed for 8 weeks with standard chow diets, high-fat diets (HFD), or high-fat diets with supplement of α-amylase inhibitor in white kidney beans (P. vulgaris extract, PVE), yogurt (Y), and PVE added yogurt (YPVE), respectively. The HFD weakened glucose tolerance and caused insulin resistance in mice, and changed the characteristics of intestinal flora. The intervention of Y, PVE, and YPVE decreased blood glucose, insulin, hyperlipidemia, and inflammatory cytokine levels in mice fed with HFD. Moreover, the YPVE could regulate the components of host intestinal microbiota toward a healthy pattern, significantly increased the metabolic-related flora Corynebacterium, Granulicatella, and Streptococcus, while it decreased Paraprevotella and Allobaculum. Thus, YPVE markedly increased functions of “Amino Acid Metabolism,” “Energy Metabolism,” “Nucleotide Metabolism,” and declined functions of “Glycan Biosynthesis and Metabolism.” Consequently, YPVE could be developed as a new functional food because of its beneficial prebiotic properties in the metabolic syndrome.

Highlights

White kidney bean is one species of Phaseolus vulgaris L., known as common bean
We detected that the α-amylase inhibitor from our testing white kidney bean original in China was a structurally uniform glycoprotein with high content of protein, which is consistent with the previous studies in other white kidney beans [21]
While in the intervention groups, the insulin resistances were suppressed and recovered to a similar level of control group in the YPVE group (Figure 2D). These results suggested that the intervention of white kidney bean extract and yogurt decreased the requirement of insulin to maintain comparable glucose concentration, significantly improved glucose tolerance, insulin sensitivity, and insulin resistance

Summary

Introduction

White kidney bean is one species of Phaseolus vulgaris L., known as common bean. It is consumed in considerable quantities around the world as a grain [1]. It is a plant native to America, especially in the Andean and Mesoamerican regions [2]. White kidney bean is a good source of high proteins (16–33%), complex carbohydrates, and some vitamins and minerals. It can be incorporated into soup and stew. The white kidney bean has been observed as a nutraceutical because it

Methods

Results

Discussion

Conclusion