Abstract
When stable flies, Stomoxys calcitrans (L.), were fed daily meals of citrated bovine blood or a glucose-saline mixture (Gatorade™), only the blood-fed flies produced cuticular hydrocarbons which are the female sex pheromone. Furthermore, only the blood diet induced sexual behavior. Male blood-fed flies displayed copulatory behavior when paired with female blood-fed flies, whereas male Gatorade flies were unresponsive to pheromone stimulation. Female Gatorade flies treated with sex pheromone extract were also attractive to male blood-fed flies, but were unreceptive to insemination despite male copulatory activity.
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