Dietary histidine requirement and physiological effects of dietary histidine deficiency in juvenile red drum Sciaenop ocellatus

Abstract

In order to develop nutritious and cost-effective diets, the amino acid requirements of cultured fish species must be quantified. Several indispensable amino acid requirements of red drum Sciaenops ocellatus have already been reported; however, its requirement for histidine (His) has yet to be quantified. The aims of the present studies were to determine the minimum dietary His requirement of juvenile red drum and the physiological effects of His deficiency. A basal diet was prepared using lyophilized red drum muscle as an intact protein (10.5% of dietary protein) supplemented with crystalline amino acids (excluding His) to provide a total of 35% crude protein (CP) in the diet. Five isonitrogenous and isoenergetic experimental diets were prepared by supplementing the basal diet (0.3g His/100g dry diet) with increasing amounts of His (0.5, 0.7, 0.9, 1.1, 1.3g/100g dry diet). These diets were fed to triplicate tanks of juvenile red drum (initial average weight of 0.98±0.08g/fish) for 6weeks in feeding trial 1 to quantify the minimum dietary His requirement. His level had significant effects on relative weight gain, feed efficiency ratio, protein efficiency ratio, and plasma free His. According to quadratic broken-line regression on relative weight gain percentage, the dietary His requirement was 0.59 (±0.15) g/100g dry diet (1.6% of CP). Feeding trial 2 was performed to further examine His deficiency in red drum. In that trial, the basal diet and a His-supplemented diet (~1.2g/100g dry diet) were fed to juvenile red drum (initial average weight of 1.53±0.08g/fish) in triplicate tanks for 8weeks. Cataracts were found in 16.7% of eyes from fish fed the basal diet while no fish in the supplemented group developed cataracts. His-supplemented fish were found to have higher erythrocyte fragility than those fed the basal diet. Based on these results, the His requirement of red drum was defined and His deficiency was observed to affect blood parameters and increase the possibility of cataract development.