Abstract
A 16-week growth study was conducted to determine the dietary folic acid requirement of juvenile abalone (initial mean weight 482.2±2.2mg). Six semi-purified diets were formulated to contain graded levels of folic acid (0.06, 1.44, 2.86, 5.88, 14.27 and 35.71mg/kg). Each diet was fed to three replicate tanks of abalone in a re-circulated water system. The results showed that the weight gain rate (WGR) was significantly increased when dietary folic acid contents increasing from 0.06 to 2.86mg/kg (P<0.05). Higher dietary folic acid (2.86–35.71mg/kg) did not result in further increase of WGR. When dietary folic acid increased from 0.06 to 5.88mg/kg, the viscera folic acid concentration (VFAC) significantly increased (P<0.05). And then, there were no significant differences in VFAC among treatments as dietary folic acid contents increased from 5.88 to 35.71mg/kg. Dietary folic acid did not significantly influence survival and the soft body compositions of abalone. Based on WGR and VFAC, broken-line analysis showed that dietary folic acid requirements of abalone were 2.62 and 5.29mg/kg, respectively.
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