Dietary Fatty Acid Profile Influences Circulating and Tissue Fatty Acid Ethanolamide Concentrations in a Tissue-specific Manner in Male Syrian Hamsters (P08-016-19)

Abstract

Abstract Objectives To elucidate the impact of diet on fatty acid ethanolamide (FAE) levels in seven different tissues/organs of male hamsters, with the hypothesis that dietary fatty acids (DFA) will act as precursors for FAE synthesis in golden Syrian male hamsters. Methods A two month feeding trial was performed, wherein hamsters (n = 105) were fed various dietary oil blends; namely, C + S, 25:75 corn oil: n9 safflower oil; F + S, 25:75 flaxseed oil: n6 safflower oil; H + DHA, 85:15 high oleic canola oil: docosahexaenoic acid; H + EPA, 85:15 high oleic canola oil: eicosapentaenoic acid; HOCO, high oleic canola oil; OO, olive oil; and RC, regular canola oil. Tissue fatty acid (FA) and FAE concentrations were assessed using GC-FID and UPLC-MS/MS, respectively. Results Results show that DFA directly influences tissue FA and FAE levels. After C18:1n9-enriched dietary treatments, marked increases (P < 0.05) were observed in duodenal C18:1n9 and oleoylethanolamide (OEA) concentrations. Overall, a weak negative association was observed between OEA concentrations and body weight (BW) at the endpoint, (r = –0.10216; P = 0.0070). Furthermore, among all tissues; namely, adipose tissue brown (ATB), adipose tissue white (ATW), brain, heart, intestine-duodenum (I-D), intestine-jejunum (I-J), and liver a negative correlation was observed between brain OEA concentrations and BW, (r = –0.22669; P = 0.0269). Conclusions DFA composition influences FA and FAE levels across all tissues. Furthermore, we summarize that brain transmits anorexic properties mediated via neuronal signalling which may contribute to the maintenance of healthy body weight and that the benefits of OEA can be enhanced by the inclusion of C18:1n9-enriched diets. Funding Sources The Natural Sciences and Engineering Research Council of Canada supported this research.