Abstract
Epidemiologic data support an association between diet and mutations in the Kirsten-ras (KRAS) gene involved in colorectal cancer (CRC) development. This study aimed to explore the associations between fat intake and KRAS mutations in codons 12 and 13 in cases of CRC in the Moroccan population. A multicenter case-series study nested in a large-scale Moroccan CRC case-control study was conducted. Among all CRC cases recruited, 151 specimens were available for the DNA mutation analysis. Logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (Cis) for KRAS mutation status according to the fat intake variables. A KRAS mutation was detected in the CRC tumor of 34.4% of the patients among whom 65.4% had a single mutation at codon 12 and 34.6% had a single mutation at codon 13. Compared to low levels of consumption, a positive association was observed between high polyunsaturated fatty acids (PUFA) consumption (>16.9 g/day) and prevalence of KRAS mutations (OR = 2.15, 95% CI = 1.01–4.59). No statistically significant associations were observed for total fat, monounsaturated fatty acids, saturated fatty acids and KRAS mutations. The results of this study suggest that PUFA may be relevant in the etiology of CRC, possibly through the generation of G > A transitions at the KRAS oncogene. Further studies are needed to verify and explain this finding.
Highlights
Colorectal cancer (CRC), characterized by the accumulation of genetic and epigenetic alterations, is one of the most prevalent types of cancer worldwide [1]
Among 1483 cases recruited in the overall case-control study (48), mutation analysis was successful in 151 cases from 154 available specimens (98%)
KRAS mutations were detected in the colorectal cancer (CRC) tumors of 34.4% (52/151) of the patients, among whom 65.4% had a single mutation at codon and 34.6% had a single mutation at codon
Summary
This study aimed to explore the associations between fat intake and KRAS mutations in codons 12 and 13 in cases of CRC in the Moroccan population. This study is aimed at exploring the associations between fat intake and KRAS mutation on codons 12 and 13 in the case of CRC risk in the Moroccan population. We aimed to determine the association between fat intake and the prevalence of KRAS mutations at codons 12 and 13 of exon 2 in colorectal carcinomas
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