Abstract
Twenty-four Holstein cows were randomly assigned to treatment sequence and fed two levels of alfalfa hay (1.0kg/100kg body weight and 2.5kg/100kg body weight) and three types of concentrate (control, 6% added tallow, and 6% added cottonseed oil). The low roughage diet increased the 18:2 serum cholesterol esters while depressing the 20:1 fraction. Feeding tallow caused a depression of 12:0, 14:0, and 14:1 on the low roughage diet. While tallow caused an increase in 16:1, 16:2, and 18:3 on both roughage groups, the increase was significant only on the high roughage group. Cottonseed oil in the diet lowered 18:3 and 20:4 on the low roughage fed animals. Tallow raised 20:1 for both roughage groups. There were no significant correlation coefficients between the fatty acids of serum cholesterol esters and milk fatty acids.
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