Abstract
A 3-factor, 3-level Box-Behnken design was used to investigate the interaction effect of dietary digestible lysine (dLys, 9.5, 10.5, 11.5g/kg), apparent metabolizable energy (AMEn, 12.77, 13.19, 13.61MJ/kg) and available P (avP, 3.0, 4.0, 5.0g/kg) levels on performance and amino acid (AA) digestibility of Ross 308 male broilers (n = 1,050) from d 14 to 34. The design consisted of 15 treatments each replicated 5 times with 12 birds per replicate. On d 34, 3 birds were sampled from each pen to collect ileal digesta (pooled per pen) to analyze AA. Response surface was fitted by first-, second-, or third-degree polynomial regressions in JMP statistical software v. 12.0.1. Feed intake (FI), weight gain (WG) and feed conversion ratio (FCR) were affected by dLys (linear and quadratic, P <0.01), AMEn (linear, P <0.01) and AMEn × avP (P <0.01). Increased dLys increased FI but increased AMEn decreased FI in the birds fed the low-avP diet. However, when the avP level in the diet was increased, FI decreased to 13MJ/kg AMEn and remained constant thereafter. Increased dLys increased WG whereas an increase in AMEn decreased WG in the birds fed the low-avP diet but had no effect on WG in those fed the high-avP diet. Increased dLys decreased FCR whereas increased AMEn decreased FCR in the birds fed the low-avP diet but had no effect on FCR in those fed the high-avP diet. Increased dLys increased breast yield percentage (linear, P <0.01 and quadratic, P <0.05) whereas increased AMEn decreased breast yield percentage (linear, P <0.01). Dietary levels of dLys or avP had positive, linear effects on apparent ileal digestibility (AID) of methionine (P <0.01) and threonine (P <0.01) but had no effect on other AA (P >0.05). These results indicate that increasing dLys levels above current industry standard would improve broiler performance irrespective of AMEn or avP levels of the diet.
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