Dietary ellagic acid supplementation attenuates intestinal damage and oxidative stress by regulating gut microbiota in weanling piglets

Abstract

Intestinal oxidative stress triggers gut microbiota dysbiosis, which is involved in the etiology of post-weaning diarrhea and enteric infections. Ellagic acid (EA) can potentially serve as an antioxidant supplement to facilitate weaning transition by improving intestinal oxidative stress and gut microbiota dysbiosis. Therefore, we aimed to investigate the effects of dietary EA supplementation on the attenuation of intestinal damage, oxidative stress, and dysbiosis of gut microbiota in weanling piglets. A total of 126 piglets were randomly assigned into 3 groups and treated with a basal diet and 2mL saline orally (Ctrl group), or the basal diet supplemented with 0.1% EA and 2mL saline orally (EA group), or the basal diet and 2mL fecal microbiota suspension from the EA group orally (FEA group), respectively, for 14d. Compared with the Ctrl group, EA group improved growth performance by increasing average daily feed intake and average daily weight gain (P<0.05) and decreasing fecal scores (P<0.05). EA group also alleviated intestinal damage by increasing the tight junction protein occludin (P<0.05), villus height, and villus height-to-crypt depth ratio (P<0.05), while decreasing intestinal epithelial apoptosis (P<0.05). Additionally, EA group enhanced the jejunum antioxidant capacity by increasing the total antioxidant capacity (P<0.01), catalase (P<0.05), and glutathione/oxidized glutathione (P<0.05), but decreased the oxidative metabolite malondialdehyde (P<0.05) compared to the Ctrl group. Compared with the Ctrl group, EA and FEA groups increased alpha diversity (P<0.05), enriched beneficial bacteria (Ruminococcaceae and Clostridium ramosum), and increased metabolites short-chain fatty acids (P<0.05). Correspondingly, FEA group gained effects comparable to those of EA group on growth performance, intestinal damage, and intestinal antioxidant capacity. In addition, the relative abundance of bacteria shifted in EA and FEA groups was significantly related to the examined indices (P<0.05). Overall, dietary EA supplementation could improve growth performance and attenuate intestinal damage and oxidative stress by regulating the gut microbiota in weanling piglets.